Check-Points

Microarray identification of carbapenemase, ESBL and AmpC genes

Check-Points
MDR CT

The clinically prevalent carbapenemase, ESBL and AmpC genes in a single test

Simultaneous detection using up to 100 specific resistance markers

High specificity and sensitivity for all species

Accuracy down to a single base pair allows distinguishing ESBL from non-ESBL for TEM and SHV

Automated analysis of results

Software database of results improves traceability and simplifies data management

How it works

1 Identification
  • The probe arms will be ligated together only if they match exactly to the template.
  • A single nucleotide mismatch will prevent ligation of the probe arms.
2 Amplification
  • Only ligated probes are amplified (and labeled).
3 Detection
  • Different probes will hybridize to different positions on the microarray through the ZIP region of the probe.
  • Hybridized amplification products are visualized by colorimetric detection.
4 Analysis
  • An image of the microarray, contained in an Array Tube, is generated using the Check-Points Tube Reader.
  • The E-Ads software automatically analyzes the presence or absence of carbapenemases, AmpCs and ESBLs as well as the resistance genes present.
Illustration

Check-Points Tube Reader & E-Ads software

  • Automatically analyzes the presence of carbapenemases, AmpC and ESBL as well as the resistance genes present.
  • Software database of results improves traceability and simplifies data management.
  • Tube Reader

    Check-Points Tube Reader including E-Ads software

  • E-Ads

    Top: E-Ads software: results summary table
    Bottom: E-Ads software: automated analysis of the microarray image

Recommended reading

Check-MDR CT103
Evaluation of a DNA microarray for the rapid detection of extended-spectrum β-lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM)

Check-MDR CT102
Evaluation of a commercial microarray to detect carbapenemase-producing Enterobacteriaceae

Equipment required

Pre-PCR
  • Thermocycler*
  • Vortex Mixer
  • Mini-centrifuge
Post-PCR
  • Thermocycler*
  • Vortex Mixer
  • Mini-centrifuge
  • Thermomixer with active cooling*
  • Check-Points Tube Reader including E-Ads software
  • Computer with USB drive and internet connection
  • Barcode Reader

Ordering information

Catalog no. Description
10-0020 Check-MDR CT101 kit, 72 reactions
10-0019 Check-MDR CT102 kit, 72 reactions
10-0021 Check-MDR CT103 kit, 24 reactions
16-0012 Check-Points Tube Reader including E-Ads software

Downloads

Download the Check-MDR CT
technical note ( .pdf)
* Contact your local representative for specifications