Check-Points

Check-Points
1 reaction per microarray Our most comprehensive assay, covering the clinically most prevalent carbapenemases, ESBLs and AmpCs in a single test

Confirm suspected multidrug resistance

Carbapenemases, ESBLs and AmpCs can reduce the susceptibility to antibiotics. Check-MDR CT103 tests isolates against a large number of resistance markers in a single assay, providing you with a comprehensive and timely answer.

Improve your epidemiological insight

Gram-negative antibiotic resistance is increasing worldwide at an alarming rate. Check-MDR CT103 allows you to stay informed on the developments with accurate gene-level information and helps creating a more complete picture by also detecting combinations of resistance genes.

Exclude outbreaks

Rapid implementation of infection control measures helps to minimize the impact of outbreaks. Use Check-MDR CT103 as an outbreak investigation tool to potentially exclude outbreaks when the genetic profile of cases differ.

Resistance markers & controls

Carbapenemases* CTX-M ESBLs TEM ESBLs vs. non-ESBL SHV ESBLs vs. non-ESBL AmpCs Controls
KPC
NDM
VIM
IMP
OXA-48		 CTX-M-1 group
CTX-M-1 like
CTX-M-15 like
CTX-M-3 like
CTX-M-32 like
CTX-M-2 group
CTX-M-8 & -25 group
CTX-M-9 group		 TEM wt
TEM E104K
TEM R164S
TEM R164C
TEM R164H
TEM G238S		 SHV wt
SHV G238S
SHV G238A
SHV E240K		 CMY I/MOX
ACC
DHA
ACT/MIR
CMY II FOX		 DNA control
Amplification control
Hybridization control
Negative control

*All known variants of KPC, NDM and OXA-48 (including OXA-181, 162, 163) reported at www.lahey.org/studies as of March 2012 are detected. VIM variants detected: VIM-1 until -6, VIM-8 until -29. IMP variants detected: IMP-1, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 15, 19, 20, 24, 25.

Check-MDR CT workflow

1

Culture
Time per step
Hands-on time per step

2

DNA extraction
Isolate total nucleic acid using automated, magnetic bead or column-based methods (not supplied).
 
 

3

Identification
Ligation-mediated PCR ensures the highly accurate identification of resistance genes.
3h
15min

4

Amplification
Only ligated probes are amplified.
1.5h
15min

5

Detection
Probes hybridize to specific locations on the microarray, contained in a Check-Points Array Tube.
1.5h
2x30min

6

Results
An image of the microarray is generated using the Check-Points Tube Reader.
30 min
30 min

Equipment required

Pre-PCR
  • Thermocycler*
  • Vortex Mixer
  • Mini-centrifuge
Post-PCR
  • Thermocycler*
  • Vortex Mixer
  • Mini-centrifuge
  • Check-Points Tube Reader including E-Ads software
  • Computer with USB drive and internet connection
  • Barcode Reader

Ordering information

Catalog no. Description
10-0020 Check-MDR CT101 kit, 72 reactions
10-0019 Check-MDR CT102 kit, 72 reactions
10-0021 Check-MDR CT103 kit, 24 reactions
16-0012 Check-Points Tube Reader including E-Ads software

Distributors

Click here to find your local distributor.

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For research use only in the US.

* Contact your local representative for specifications